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Addgene

pVH016
(Plasmid #80400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1
  • Backbone size w/o insert (bp) 6100
  • Total vector size (bp) 9365
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ffGFP-3xFLAG
  • Insert Size (bp)
    777
  • Tag / Fusion Protein
    • C-terminal 3xFLAG tag

Gene/Insert 2

  • Gene/Insert name
    CusR
  • Insert Size (bp)
    836
  • Entrez Gene
    cusR (a.k.a. b0571, ECK0563, JW0560, silR, ylcA)
  • Tag / Fusion Protein
    • C-terminal LZx domain

Gene/Insert 3

  • Gene/Insert name
    AraC
  • Insert Size (bp)
    879
  • Entrez Gene
    araC (a.k.a. b0064, ECK0065)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVH016 was a gift from Richard Murray (Addgene plasmid # 80400 ; http://n2t.net/addgene:80400 ; RRID:Addgene_80400)

  • For your References section:

    Design and implementation of a biomolecular concentration tracker. Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM. ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. Epub 2014 May 15. 10.1021/sb500024b PubMed 24847683
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