pWW2149
(Plasmid
#80392)
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PurposeContains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80392 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A
- Backbone size w/o insert (bp) 2300
- Total vector size (bp) 4973
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTaz Histidine Kinase
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Alt nameTaz HK
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Insert Size (bp)1449
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Tag
/ Fusion Protein
- Fused by GS linkers to 4 SH3 domains
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byOriginally made by Weston Whitaker from John Dueber's lab at Berkeley
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWW2149 was a gift from Richard Murray (Addgene plasmid # 80392 ; http://n2t.net/addgene:80392 ; RRID:Addgene_80392) -
For your References section:
Design and implementation of a biomolecular concentration tracker. Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM. ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. Epub 2014 May 15. 10.1021/sb500024b PubMed 24847683