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Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintaince
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGreenPuro
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Backbone manufacturerSBI
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecopGFP-T2A-Puromycine
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN(CMV-copGFP-Puro-SMAR) was a gift from Magnus Essand (Addgene plasmid # 80391 ; http://n2t.net/addgene:80391 ; RRID:Addgene_80391) -
For your References section:
Safe engineering of CAR T cells for adoptive cell therapy of cancer using long-term episomal gene transfer. Jin C, Fotaki G, Ramachandran M, Nilsson B, Essand M, Yu D. EMBO Mol Med. 2016 May 6. pii: e201505869. doi: 10.15252/emmm.201505869. 10.15252/emmm.201505869 PubMed 27189167