pMALac-OMA-1(1-182)
(Plasmid
#80350)
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PurposeExpresses OMA-1 (1-182) in bacteria for protein purification purposes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMAL-ac
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOMA-1
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SpeciesC. elegans (nematode)
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MutationEncodes AA residues 1-182
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Entrez Geneoma-1 (a.k.a. CELE_C09G9.6)
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Tag
/ Fusion Protein
- 6xHis-MBP-TEV (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMALac-OMA-1(1-182) was a gift from Sean Ryder (Addgene plasmid # 80350 ; http://n2t.net/addgene:80350 ; RRID:Addgene_80350) -
For your References section:
RNA recognition by the Caenorhabditis elegans oocyte maturation determinant OMA-1. Kaymak E, Ryder SP. J Biol Chem. 2013 Oct 18;288(42):30463-72. doi: 10.1074/jbc.M113.496547. Epub 2013 Sep 6. 10.1074/jbc.M113.496547 PubMed 24014033