pAAV2.5-THP-dsRed2
(Plasmid #80335)

• Purpose: Fluorescent Reporter for Dopaminergic Neuron Differentiation
• Depositing Lab: Kwang-Soo Kim
• Publication: Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 10.1038/gt.2008.148. Epub 2008 Sep 18.

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Backbone

• Vector backbone: pAAV2.5
• Backbone size w/o insert (bp): 4244
• Total vector size (bp): 6744
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rat TH promoter
• Species: R. norvegicus (rat)
• Insert Size (bp): 2500
• GenBank ID: AF014956
• Entrez Gene: Th (a.k.a. The)
• Promoter: rat Tyrosine hydroxylase

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sfi I (destroyed during cloning)
• 3′ cloning site: Alu I (not destroyed)
• 5′ sequencing primer: GAGTGGCCAACTCCATCACT
• 3′ sequencing primer: GAGTTCATGCGCTTCAAGGT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry