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pDrVSP(C302S)-IRES2-EGFP
(Plasmid #80331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clonetech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Daino rerio voltage-sensing phosphatase
  • Alt name
    Dr-VSP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1536
  • Mutation
    C302S
  • GenBank ID
    AB308476
  • Entrez Gene
    tpte (a.k.a. tpip, vsp, wu:fd20e11, wu:fi24b06)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer GCAGAGCTGGTTTAGTGAACCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDrVSP(C302S)-IRES2-EGFP was a gift from Yasushi Okamura (Addgene plasmid # 80331 ; http://n2t.net/addgene:80331 ; RRID:Addgene_80331)

  • For your References section:

    Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish voltage-sensing phosphatases. Hossain MI, Iwasaki H, Okochi Y, Chahine M, Higashijima S, Nagayama K, Okamura Y. J Biol Chem. 2008 Jun 27;283(26):18248-59. doi: 10.1074/jbc.M706184200. Epub 2008 Mar 28. 10.1074/jbc.M706184200 PubMed 18375390
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