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pFUPI hT5Cyp-H126Q
(Plasmid #80281)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80281 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUPI
  • Backbone size w/o insert (bp) 10306
  • Total vector size (bp) 11781
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRIM5 CypA fusion
  • Alt name
    tripartite motif containing 5
  • Alt name
    peptidylprolyl isomerase A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1475
  • Mutation
    H126Q mutation in CypA
  • Entrez Gene
    TRIM5 (a.k.a. RNF88, TRIM5alpha)
  • Entrez Gene
    PPIA (a.k.a. CYPA, CYPH, HEL-S-69p)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUPI hT5Cyp-H126Q was a gift from Jeremy Luban (Addgene plasmid # 80281 ; http://n2t.net/addgene:80281 ; RRID:Addgene_80281)

  • For your References section:

    Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components. Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J. J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub 2009 Sep 8. 10.1172/JCI39354 PubMed 19741300
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