pFUPI
(Plasmid
#80279)
-
PurposeA bicistronic lentiviral vector used to stably express cDNAs.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 80279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFUW
- Backbone size w/o insert (bp) 9206
- Total vector size (bp) 10306
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePuro resistance cassette, IRES, cloning site
-
Insert Size (bp)1100
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFUPI was a gift from Jeremy Luban (Addgene plasmid # 80279 ; http://n2t.net/addgene:80279 ; RRID:Addgene_80279) -
For your References section:
Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components. Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J. J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub 2009 Sep 8. 10.1172/JCI39354 PubMed 19741300