pcDNA5 FRT/TO Plk4 KD-mCherry
(Plasmid #80270)

• Purpose: mammalian expression plasmid for kinase dead Plk4
• Depositing Lab: Andrew Holland
• Publication: Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.

Backbone

• Vector backbone: pcDNA5 FRT/TO
• Backbone size w/o insert (bp): 5807
• Total vector size (bp): 8717
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Plk4
• Alt name: Polo like kinase 4
• Species: H. sapiens (human)
• Insert Size (bp): 2910
• Mutation: D154A kinase dead
• Entrez Gene: PLK4 (a.k.a. MCCRP2, SAK, STK18)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry