pcDNA5 FRT/TO MycLAP-STIL ∆CC
(Plasmid
#80268)
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Purposemammalian expression plasmid for MycLAP-STIL coiled-coil deletion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80268 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5 FRT/TO
- Backbone size w/o insert (bp) 6126
- Total vector size (bp) 9894
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTIL
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SpeciesH. sapiens (human)
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Insert Size (bp)3861
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Mutationmissing conserved coiled-coil (amino acids 721-747)
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Entrez GeneSTIL (a.k.a. MCPH7, SIL)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc-GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5 FRT/TO MycLAP-STIL ∆CC was a gift from Andrew Holland (Addgene plasmid # 80268 ; http://n2t.net/addgene:80268 ; RRID:Addgene_80268) -
For your References section:
Binding of STIL to Plk4 activates kinase activity to promote centriole assembly. Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ. J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088. 10.1083/jcb.201502088 PubMed 26101219
Map uploaded by the depositor.
