pFastBac GST-Plk4
(Plasmid
#80264)
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Purposebaculovirus creation plasmid for GST-Plk4
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80264 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBac
- Backbone size w/o insert (bp) 5421
- Total vector size (bp) 8331
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePlk4
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Alt namePolo-like kinase 4
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SpeciesH. sapiens (human)
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Insert Size (bp)2910
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Entrez GenePLK4 (a.k.a. MCCRP2, SAK, STK18)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac GST-Plk4 was a gift from Andrew Holland (Addgene plasmid # 80264 ; http://n2t.net/addgene:80264 ; RRID:Addgene_80264) -
For your References section:
Binding of STIL to Plk4 activates kinase activity to promote centriole assembly. Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ. J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088. 10.1083/jcb.201502088 PubMed 26101219
Map uploaded by the depositor.
