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35S-frGC155-MXMT-NosT
(Plasmid #80172)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80172 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBRN169-MXMT
  • Backbone manufacturer
    Kodama and Wada 2009
  • Backbone size w/o insert (bp) 3465
  • Total vector size (bp) 4854
  • Modifications to backbone
    PCR-amplied DNA fragments (frGC) for BiFC fragments were subcloned into the NcoI/BsrGI site of pBRN169-MXMT vector.
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    frGFP(156-238)
  • Alt name
    frGC155
  • Species
    Synthetic
  • Insert Size (bp)
    258
  • Promoter CaMV 35S promoter
  • Tag / Fusion Protein
    • frGC155 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoⅠ (not destroyed)
  • 3′ cloning site BsrGⅠ (not destroyed)
  • 5′ sequencing primer GGATCCATGGTGGACAAGCAGAAGAACGGC
  • 3′ sequencing primer TTACTTGTACAGCTCGTCCATGCCGAGAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    35S-frGC155-MXMT-NosT was a gift from Yutaka Kodama (Addgene plasmid # 80172 ; http://n2t.net/addgene:80172 ; RRID:Addgene_80172)

  • For your References section:

    In planta comparative analysis of improved green fluorescent proteins with reference to fluorescence intensity and bimolecular fluorescence complementation ability. Fujii Y, Kodama Y. Plant Biotech. 2015; 32(1): 81-87. 10.5511/plantbiotechnology.15.0120a
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