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PurposeMinigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80169 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBluescript KS+
- Total vector size (bp) 5724
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesegments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
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SpeciesG. gallus (chicken)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RHCglo was a gift from Thomas Cooper (Addgene plasmid # 80169 ; http://n2t.net/addgene:80169 ; RRID:Addgene_80169) -
For your References section:
Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing. Singh G, Cooper TA. Biotechniques. 2006 Aug;41(2):177-81. 10.2144/000112208 PubMed 16925019