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Addgene

pEIAV-iSyn-eGFPW
(Plasmid #80161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EIAV
  • Backbone manufacturer
    John Olsen UNC
  • Backbone size w/o insert (bp) 4876
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720
  • Promoter iSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer hSyn1pro-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    backbone from John Olsen UNC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEIAV-iSyn-eGFPW was a gift from Edward Callaway (Addgene plasmid # 80161 ; http://n2t.net/addgene:80161 ; RRID:Addgene_80161)

  • For your References section:

    Optical control of retrogradely infected neurons using drug-regulated "TLoop" lentiviral vectors. Cetin A, Callaway EM. J Neurophysiol. 2014 May;111(10):2150-9. doi: 10.1152/jn.00495.2013. Epub 2014 Feb 26. 10.1152/jn.00495.2013 PubMed 24572099
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