pEIAV-TLoop-Chr2-YFP
(Plasmid #80160)

• Purpose: Expression in mammalian cells
• Depositing Lab: Edward Callaway
• Publication: Cetin et al J Neurophysiol. 2014 May;111(10):2150-9. doi: 10.1152/jn.00495.2013. Epub 2014 Feb 26.

Backbone

• Vector backbone: EIAV
• Backbone manufacturer: John Olsen UNC
• Backbone size w/o insert (bp): 4876
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chr2
• Species: Chlamydomonas
• Insert Size (bp): 1655
• Mutation: H134R
• Promoter: CMV TetOx7
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: backbone from John Olsen UNC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry