Banshee RunxDN mCherry
(Plasmid
#80158)
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Purposeretroviral expression of Runx1DN and mCherry
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBanshee
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Backbone manufacturerDr. John Rossi, Beckman Research Institute of the City of Hope, CA
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRunx1
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SpeciesM. musculus (mouse)
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MutationDN--truncated version of the Runx1 protein containing only the DNA binding domain.
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Entrez GeneRunx1 (a.k.a. AML1, CBF-alpha-2, Cbfa2, Pebp2a2, Pebpa2b)
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Tags
/ Fusion Proteins
- HA (C terminal on insert)
- IRES-mCherry (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GCCCTTTGTACACCCTAAGCCT
- 3′ sequencing primer mCherry-R or TCAAGAAGACAGGGCCAGGTTT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Banshee RunxDN mCherry was a gift from Ellen Rothenberg (Addgene plasmid # 80158 ; http://n2t.net/addgene:80158 ; RRID:Addgene_80158)