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Addgene

Banshee shTCF1 mCherry
(Plasmid #80154)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80154 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Banshee
  • Backbone manufacturer
    Dr. John Rossi, Beckman Research Institute of the City of Hope, CA
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCF1
  • gRNA/shRNA sequence
    GCCACGAGTCTGAACAATAAT TTCAAGAGAATTATTGTTTAGACTTGTGGC

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AGACAGGATATCAGTGGTCCAGG
  • 3′ sequencing primer mCherry-R or CCTGTTTGGCCCATATTCTGCT
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Banshee shTCF1 mCherry was a gift from Ellen Rothenberg (Addgene plasmid # 80154 ; http://n2t.net/addgene:80154 ; RRID:Addgene_80154)