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Addgene

PU.1-ets-IRES-mCherry
(Plasmid #80141)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    IRES-mCherry
  • Backbone manufacturer
    Rothernberg lab, Addgene plasmid 80139
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PU.1
  • Species
    M. musculus (mouse)
  • Mutation
    ets = PU.1 DNA binding domain (amino acids 159-262)
  • Entrez Gene
    Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
  • Tag / Fusion Protein
    • IRES-mCherry (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PU.1-ets-IRES-mCherry was a gift from Ellen Rothenberg (Addgene plasmid # 80141 ; http://n2t.net/addgene:80141 ; RRID:Addgene_80141)

  • For your References section:

    Positive feedback between PU.1 and the cell cycle controls myeloid differentiation. Kueh HY, Champhekar A, Nutt SL, Elowitz MB, Rothenberg EV. Science. 2013 Aug 9;341(6146):670-3. doi: 10.1126/science.1240831. Epub 2013 Jul 18. 10.1126/science.1240831 PubMed 23868921
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