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Purpose(Empty Backbone) empty retroviral vector with IRES-mCherry
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80139 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV2.2
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Vector typeMammalian Expression, Retroviral
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Tag
/ Fusion Protein
- IRES-mCherry (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IRES-mCherry was a gift from Ellen Rothenberg (Addgene plasmid # 80139 ; http://n2t.net/addgene:80139 ; RRID:Addgene_80139) -
For your References section:
Positive feedback between PU.1 and the cell cycle controls myeloid differentiation. Kueh HY, Champhekar A, Nutt SL, Elowitz MB, Rothenberg EV. Science. 2013 Aug 9;341(6146):670-3. doi: 10.1126/science.1240831. Epub 2013 Jul 18. 10.1126/science.1240831 PubMed 23868921
