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Addgene

Id2 Ngfr
(Plasmid #80131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    NGFR
  • Backbone manufacturer
    Warren Pear (Addgene plasmid # 27489)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Id2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    417
  • Entrez Gene
    Id2 (a.k.a. Idb2, bHLHb26)
  • Tag / Fusion Protein
    • IRES-Ngfr (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer pLXSN5'
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Id2 Ngfr was a gift from Ellen Rothenberg (Addgene plasmid # 80131 ; http://n2t.net/addgene:80131 ; RRID:Addgene_80131)

  • For your References section:

    Architecture of a lymphomyeloid developmental switch controlled by PU.1, Notch and Gata3. Del Real MM, Rothenberg EV. Development. 2013 Mar;140(6):1207-19. doi: 10.1242/dev.088559. 10.1242/dev.088559 PubMed 23444353
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