pWUR 1+2 tetO mut89
(Plasmid #80102)

• Purpose: Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2
• Depositing Lab: George Church
• Publication: Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Backbone

• Vector backbone: pCDF-1b
• Total vector size (bp): 7356
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas1
• Entrez Gene: cas1 (a.k.a. b2755, ECK2750, ygbT)

Gene/Insert 2

• Gene/Insert name: Cas2
• Entrez Gene: cas2 (a.k.a. b2754, ECK2749, ygbF)

Gene/Insert 3

• Gene/Insert name: Cas1
• Mutation: Q24H, P202Q, G241D, E276D, L297Q
• Entrez Gene: cas1 (a.k.a. b2755, ECK2750, ygbT)

Gene/Insert 4

• Gene/Insert name: Cas2
• Entrez Gene: cas2 (a.k.a. b2754, ECK2749, ygbF)

Resource Information

• Supplemental Documents:
  • pWUR 1+2 tetO mut89
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry