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Purpose(Empty Backbone) An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCB301
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Vector typePlant Expression
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Selectable markersGentamicin ; Rifampicin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsIt is probably best to do a large scale prep (eg 500 mls culture), because plasmid is fairly low copy.
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Copy numberLow Copy
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJL-TRBO was a gift from John Lindbo (Addgene plasmid # 80082 ; http://n2t.net/addgene:80082 ; RRID:Addgene_80082) -
For your References section:
TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Lindbo JA. Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24. 10.1104/pp.107.106377 PubMed 17720752
