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Addgene

pMSCV-Flag-Mm Ezh2 Y641F
(Plasmid #80077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCVhygro-F-EZH2
  • Backbone manufacturer
    Addgene distributes
  • Total vector size (bp) 9220
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Flag-EZH2 Y641F
  • Alt name
    KMT6A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2268
  • Mutation
    Y641F, R342R, N393N
  • GenBank ID
    NP_031997.2
  • Entrez Gene
    Ezh2 (a.k.a. Enx-1, Enx1h, KMT6, mKIAA4065)
  • Tag / Fusion Protein
    • 5' FLAG (N terminal on backbone)

Gene/Insert 2

  • Gene/Insert name
    Hygromycin phosphotransferase
  • Alt name
    hpt
  • Insert Size (bp)
    1026

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene (Kai Ge original depositor)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-Flag-Mm Ezh2 Y641F was a gift from Phillip Abbosh (Addgene plasmid # 80077 ; http://n2t.net/addgene:80077 ; RRID:Addgene_80077)
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