pcDNA5-FRT-TO-EGFP-AID
(Plasmid #80075)

• Purpose: Mammalian expression vector for N-terminal EGFP-AID tagging
• Depositing Lab: Andrew Holland
• Publication: pBabe Neo OsTIR1-9Myc (unpublished)

Backbone

• Vector backbone: pcDNA5-FRT-TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5161
• Total vector size (bp): 6574
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-AID
• Alt name: Auxin Inducible Degron
• Alt name: Enhanced Green Fluorescent Protein
• Insert Size (bp): 1413
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (N terminal on backbone)
  • AID (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV Fow
• 3′ sequencing primer: BGH Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The AID was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry