pBabe Puro osTIR1-9Myc
(Plasmid #80074)

• Purpose: Retroviral vector for stable expression of osTIR1-9Myc in mammalian cell lines
• Depositing Lab: Andrew Holland
• Publication: Lambrus et al Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5.

Backbone

• Vector backbone: pBabe
• Backbone size w/o insert (bp): 5213
• Total vector size (bp): 7307
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OsTIR1
• Alt name: TRANSPORT INHIBITOR RESPONSE 1
• Species: O. sativa
• Insert Size (bp): 2094
• Entrez Gene: TIR1 (a.k.a. AT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1)
• Promoter: Gag
• Tag / Fusion Protein:
  • 9 x Myc (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBabe Fow
• 3′ sequencing primer: pBabe Rev

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The osTIR1-9Myc was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).