GFP-BLM DM
(Plasmid #80071)

• Purpose: Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations
• Depositing Lab: Nathan Ellis
• Publication: Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-65. Epub 2005 Apr 13.

Backbone

• Vector backbone: EGFP-C1
• Backbone manufacturer: CLONTECH
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 9112
• Modifications to backbone: Inserted at Sal1 site
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BLM
• Species: H. sapiens (human)
• Mutation: K317R (AAA>AGA) and K331R (AAA>AGA)
• GenBank ID: U39817.1 NP_000048.1
• Entrez Gene: BLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
• Tag / Fusion Protein:
  • GFP-BLM (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: not sure
• 3′ sequencing primer: not sure

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry