GFP-BLM
(Plasmid #80070)

• Purpose: Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)
• Depositing Lab: Nathan Ellis
• Publication: Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98.

Backbone

• Vector backbone: EGFP-C1
• Backbone manufacturer: CLONTECH
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 9112
• Modifications to backbone: SalI cloning site duplicated. No other changes
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BLM
• Species: H. sapiens (human)
• Mutation: If correct ID, the cDNA is from HeLa and has characteristic African-ancestry nt
• GenBank ID: U39817.1 NP_000048.1
• Entrez Gene: BLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: not sure
• 3′ sequencing primer: not sure

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry