Skip to main content
Addgene

pCMV-EPN-01(ΔI)
(Plasmid #80048)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80048 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV(WT)
  • Backbone manufacturer
    ...
  • Backbone size w/o insert (bp) 5478
  • Total vector size (bp) 6305
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EPN-01(L33R)
  • Species
    Synthetic
  • Insert Size (bp)
    849
  • Mutation
    L33R
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the L33R mutation is numbered according to the original I3-01 protein and is L50R when numbered according to EPN-01.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-EPN-01(ΔI) was a gift from Wesley Sundquist (Addgene plasmid # 80048 ; http://n2t.net/addgene:80048 ; RRID:Addgene_80048)

  • For your References section:

    Designed proteins induce the formation of nanocage-containing extracellular vesicles. Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI. Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30. 10.1038/nature20607 PubMed 27919066
Related items: