pmCherry-Vinculin
(Plasmid
#80024)
-
PurposeMammalian expression of mCherry-vinculin
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80024 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEGFP
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namevinculin
-
SpeciesG. gallus (chicken)
-
Entrez GeneVCL (a.k.a. VINC1)
- Promoter CMV
-
Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer mCherry-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pmCherry-Vinculin was a gift from Chinten James Lim (Addgene plasmid # 80024 ; http://n2t.net/addgene:80024 ; RRID:Addgene_80024) -
For your References section:
Two modes of integrin activation form a binary molecular switch in adhesion maturation. Lee HS, Anekal P, Lim CJ, Liu CC, Ginsberg MH. Mol Biol Cell. 2013 May;24(9):1354-62. doi: 10.1091/mbc.E12-09-0695. Epub 2013 Mar 6. 10.1091/mbc.E12-09-0695 PubMed 23468527