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PurposeMammalian expression of human integrin alpha4
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameintegrin alpha4
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Alt nameintegrin subunit alpha 4
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Alt nameITGA4
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SpeciesH. sapiens (human)
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Entrez GeneITGA4 (a.k.a. CD49D, IA4)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-alpha4 wt was a gift from Chinten James Lim (Addgene plasmid # 80016 ; http://n2t.net/addgene:80016 ; RRID:Addgene_80016) -
For your References section:
The membrane-proximal KXGFFKR motif of alpha-integrin mediates chemoresistance. Liu CC, Leclair P, Yap SQ, Lim CJ. Mol Cell Biol. 2013 Nov;33(21):4334-45. doi: 10.1128/MCB.00580-13. Epub 2013 Sep 3. 10.1128/MCB.00580-13 PubMed 24001772