pC4EN-F1-mGAF670 (FKBP-mGAF670)
(Plasmid
#80003)
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PurposemiRFP670 GAF domain fused to FKBP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepC4EN-F1
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Backbone manufacturerARIAD Pharmaceuticals, Inc.
- Backbone size w/o insert (bp) 1961
- Total vector size (bp) 2900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFKBP
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Insert Size (bp)939
- Promoter CMV
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Tag
/ Fusion Protein
- GAF domain of miRFP670 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ctcttcggccagcgaattc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC4EN-F1-mGAF670 (FKBP-mGAF670) was a gift from Vladislav Verkhusha (Addgene plasmid # 80003 ; http://n2t.net/addgene:80003 ; RRID:Addgene_80003) -
For your References section:
Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV. Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405. 10.1038/ncomms12405 PubMed 27539380
Map uploaded by the depositor.
