pVimentin-miRFP703
(Plasmid
#79996)
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PurposeVimentin labeling with near-infrared fluorescent protein miRFP703
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepN1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3970
- Total vector size (bp) 6337
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVimentin
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Alt nameNM_003380.3
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SpeciesH. sapiens (human)
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Insert Size (bp)2367
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GenBank IDNM_003380.3
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Entrez GeneVIM
- Promoter CMV
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Tag
/ Fusion Protein
- miRFP703 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVimentin-miRFP703 was a gift from Vladislav Verkhusha (Addgene plasmid # 79996 ; http://n2t.net/addgene:79996 ; RRID:Addgene_79996) -
For your References section:
Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV. Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405. 10.1038/ncomms12405 PubMed 27539380
Map uploaded by the depositor.
