pLifeAct-miRFP703
(Plasmid #79993)

• Purpose: F-Actin labeling with near-infrared fluorescent protein miRFP703
• Depositing Lab: Vladislav Verkhusha
• Publication: Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3936
• Total vector size (bp): 4956
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lifeact
• Alt name: the first 17 amino acids of Abp140
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1020
• Promoter: CMV
• Tag / Fusion Protein:
  • miRFP703 (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry