pKeratin-miRFP703
(Plasmid #79990)

• Purpose: Keratin labeling with near-infrared fluorescent protein miRFP703
• Depositing Lab: Vladislav Verkhusha
• Publication: Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3933
• Total vector size (bp): 6222
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Keratin
• Alt name: Keratin18
• Alt name: NM_199187.1
• Species: H. sapiens (human)
• Insert Size (bp): 2289
• GenBank ID: NM_001834.2
• Entrez Gene: KRT18 (a.k.a. CK-18, CYK18, K18)
• Promoter: CMV
• Tag / Fusion Protein:
  • miRFP703 (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry