pBAD/His-miRFP709
(Plasmid #79986)

• Purpose: Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid
• Depositing Lab: Vladislav Verkhusha
• Publication: Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.

Backbone

• Vector backbone: pBAD/His-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3999
• Total vector size (bp): 4947
• Modifications to backbone: Several amino acids between His-tag and miRFP709 were removed.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: miRFP709
• Species: Synthetic
• Insert Size (bp): 948
• Promoter: araB

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry