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Addgene

pNPK-NLS-Vika
(Plasmid #79969)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79969 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNPK
  • Total vector size (bp) 6005
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human codon-optimized Vika sequence, NLS tagged
  • Species
    Synthetic; derived from Vibrio coralliilyticus ATCC BAA-450
  • Insert Size (bp)
    1110
  • GenBank ID
    NZ_ACZN01000014.1 ZP_05884863
  • Promoter PGK
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GCTGTTCTCCTCTTCCTC
  • 3′ sequencing primer CTCTGTAGGTAGTTTGTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNPK-NLS-Vika was a gift from Frank Buchholz (Addgene plasmid # 79969 ; http://n2t.net/addgene:79969 ; RRID:Addgene_79969)

  • For your References section:

    Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system. Karimova M, Abi-Ghanem J, Berger N, Surendranath V, Pisabarro MT, Buchholz F. Nucleic Acids Res. 2013 Jan;41(2):e37. doi: 10.1093/nar/gks1037. Epub 2012 Nov 9. 10.1093/nar/gks1037 PubMed 23143104
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