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Addgene

pMA7
(Plasmid #79967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Backbone size w/o insert (bp) 4542
  • Total vector size (bp) 6163
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Lambda Red recombinase beta subunit
  • Alt name
    beta
  • Species
    Escherichia coli (EcNR1)
  • GenBank ID
  • Promoter pBAD

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    DNA adenine methylase
  • Alt name
    dam
  • Species
    Escherichia coli K-12 MG1655
  • GenBank ID
  • Promoter pBAD

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA7 was a gift from Morten Sommer (Addgene plasmid # 79967 ; http://n2t.net/addgene:79967 ; RRID:Addgene_79967)

  • For your References section:

    Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects. Lennen RM, Nilsson Wallin AI, Pedersen M, Bonde M, Luo H, Herrgard MJ, Sommer MO. Nucleic Acids Res. 2016 Feb 29;44(4):e36. doi: 10.1093/nar/gkv1090. Epub 2015 Oct 22. 10.1093/nar/gkv1090 PubMed 26496947
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