pCMV-erHRP(N175S mutant)
(Plasmid
#79909)
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PurposeerHRP(N175S mutant) pCMV backbone
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameerHRP(N175S mutant)
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Alt nameHorseradish peroxidase (ER retention signal, N175S mutant)
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SpeciesSynthetic
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MutationN175S mutation
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GenBank IDKU0504630
- Promoter CMV
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Tag
/ Fusion Protein
- HA tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-erHRP(N175S mutant) was a gift from Joshua Sanes (Addgene plasmid # 79909 ; http://n2t.net/addgene:79909 ; RRID:Addgene_79909) -
For your References section:
Reconstruction of genetically identified neurons imaged by serial-section electron microscopy. Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR. Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015. 10.7554/eLife.15015 PubMed 27383271
Map uploaded by the depositor.
