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PurposeAdeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAPEX2NES
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Alt nameAPEX2 nuclear export signal
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SpeciesSynthetic
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer WPRE-R
- 3′ sequencing primer pCAG-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAPEX2NES was from Alice Ting (MIT)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-CAG-DIO-APEX2NES was a gift from Joshua Sanes (Addgene plasmid # 79907 ; http://n2t.net/addgene:79907 ; RRID:Addgene_79907) -
For your References section:
Reconstruction of genetically identified neurons imaged by serial-section electron microscopy. Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR. Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015. 10.7554/eLife.15015 PubMed 27383271