AAV-CAG-DIO-erHRP(N175S mutant)
(Plasmid
#79906)
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PurposeAdeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameerHRP(N175S mutant)
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Alt nameHorseradish peroxidase (ER retention signal, N175S mutant)
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SpeciesSynthetic
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MutationN175S mutation
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GenBank IDKU0504630
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Tag
/ Fusion Protein
- HA tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-CAG-DIO-erHRP(N175S mutant) was a gift from Joshua Sanes (Addgene plasmid # 79906 ; http://n2t.net/addgene:79906 ; RRID:Addgene_79906) -
For your References section:
Reconstruction of genetically identified neurons imaged by serial-section electron microscopy. Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR. Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015. 10.7554/eLife.15015 PubMed 27383271