BGGLO QPCR PLASMID
(Plasmid
#79905)
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PurposeEncodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79905 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1TOPO Vector
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3931
- Total vector size (bp) 4313
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Modifications to backbonePartial HBB and HBG gene inserted as QPCR standard
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman partial HBG and HBB gene
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SpeciesH. sapiens (human)
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Insert Size (bp)382
- Promoter No promoter
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BGGLO QPCR PLASMID was a gift from Jeffery Miller (Addgene plasmid # 79905 ; http://n2t.net/addgene:79905 ; RRID:Addgene_79905) -
For your References section:
A signaling mechanism for growth-related expression of fetal hemoglobin. Bhanu NV, Trice TA, Lee YT, Miller JL. Blood. 2004 Mar 1;103(5):1929-33. Epub 2003 Oct 30. 10.1182/blood-2003-05-1624 PubMed 14592835
Map uploaded by the depositor.
