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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2
(Plasmid #79902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Plasmid #68375)
  • Vector type
    Mammalian Expression, CRISPR, TALEN
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFLAG_Twin_Strep_EZH2
  • Species
    H. sapiens (human)
  • Promoter PGK1
  • Tag / Fusion Protein
    • 3xFLAG_Twin_Strep (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use in combination with eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Plasmid #79888)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2 was a gift from Yannick Doyon (Addgene plasmid # 79902 ; http://n2t.net/addgene:79902 ; RRID:Addgene_79902)

  • For your References section:

    A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. 10.1016/j.celrep.2015.09.009 PubMed 26456817
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