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Addgene

EZH2-TALEN_Left_No_FLAG
(Plasmid #79899)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79899 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    JDS78
  • Vector type
    Mammalian Expression, TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EZH2-TALEN_Left_No_FLAG

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is derived from TAL2362 (Plasmid #36775) from Keith Joung's lab. The 3x FLAG sequence was removed to prevent interference of the tag in western blot and immunoprecipitation applications.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EZH2-TALEN_Left_No_FLAG was a gift from Yannick Doyon (Addgene plasmid # 79899 ; http://n2t.net/addgene:79899 ; RRID:Addgene_79899)
  • For your References section:

    A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. 10.1016/j.celrep.2015.09.009 PubMed 26456817