FancF_N_FS_v2_Donor
(Plasmid
#79891)
-
Purpose(Empty Backbone) N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepUC19
-
Vector typeCRISPR
-
Tag
/ Fusion Protein
- 3xFLAG_Twin_Strep
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Donor plasmid for tagging FANCF at its N-terminus. Use in combination with an untagged human-codon optimized Cas9 nickase (Cas9 D10A) vector and the following gRNA expression vectors: (i) SP_gRNA_pUC19_N_FancF_Left (Plasmid #79892) and (ii) pDR348 FANCF (Plasmid #47510).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FancF_N_FS_v2_Donor was a gift from Yannick Doyon (Addgene plasmid # 79891 ; http://n2t.net/addgene:79891 ; RRID:Addgene_79891) -
For your References section:
A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. 10.1016/j.celrep.2015.09.009 PubMed 26456817
Map uploaded by the depositor.
