pWUKI 1+2
(Plasmid #79879)

• Purpose: Encodes Cas1+2 and a CRISPR array
• Depositing Lab: George Church
• Publication: Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Backbone

• Vector backbone: pCDF-1b
• Total vector size (bp): 6068
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas1
• Species: E. coli
• GenBank ID: NP_417235.1
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CACGCGGTTGGGAATGTAAT

Gene/Insert 2

• Gene/Insert name: Cas2
• Species: E. coli
• GenBank ID: NP_417234.2
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: CACTGTTGTACCGAAAGCTTT
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Gene/Insert 3

• Gene/Insert name: CRISPR Array
• Species: E. coli (K12)

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCGGCTCGTATGTTGTGTGG

Resource Information

• Supplemental Documents:
  • pWUKI 1+2 GenBank
• A portion of this plasmid was derived from a plasmid made by: Based on Plasmid #72676 from Udi Qimron.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry