pCAG_EGFP-RandE3
(Plasmid #79870)

• Purpose: Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.
• Depositing Lab: Don Arnold
• Publication: Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894.

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 4113
• Total vector size (bp): 7157
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GFP-RandE3
• Species: R. norvegicus (rat), Synthetic
• Insert Size (bp): 1302
• Promoter: Beta-actin
• Tags / Fusion Proteins:
  • GFP (N terminal on insert)
  • HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: TCCTACAGCTCCTGG
• 3′ sequencing primer: GTAAGACCAATAGGTGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry