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Addgene

pTRE-Tight-Rluc8
(Plasmid #79844)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79844 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTREtight2
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 3800
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rluc8
  • Insert Size (bp)
    900

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    A Rluc8 gene encoding modified luciferase from Renilla reniformis was amplified from a Nano-lantern/pcDNA3 plasmid (Addgene #51970)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reporter plasmid for expression of a Rluc8 behind TRE-Tight tetracycline regulatable promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE-Tight-Rluc8 was a gift from Vladislav Verkhusha (Addgene plasmid # 79844 ; http://n2t.net/addgene:79844 ; RRID:Addgene_79844)

  • For your References section:

    A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Kaberniuk AA, Shemetov AA, Verkhusha VV. Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864. 10.1038/nmeth.3864 PubMed 27159085
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