pT2/SVNeo-103
(Plasmid #79841)

• Purpose: Expresses RpBphP1-mCherry-TetR under CMVd1 promoter
• Depositing Lab: Vladislav Verkhusha
• Publication: Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.

Backbone

• Vector backbone: pT2/SVNeo Addgene #26553
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 9500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RpBphP1-mCherry-Intersectin1 DHPH domain
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 3700
• GenBank ID: NCBI Gene rpa1537 KX063613
• Promoter: CMVd1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: taatacgactcactatagg
• 3′ sequencing primer: ggaaacagctatgaccatg

Resource Information

• Supplemental Documents:
  • pT2/SVNeo-103
• A portion of this plasmid was derived from a plasmid made by: A RpBphP1 gene of R. palustris (NCBI Gene rpa1537) was provided by E. Giraud (Institute for Research and Development, France).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

New paper providing detailed protocol for using this plasmid- https://www.nature.com/articles/nprot.2018.022