pKA-144
(Plasmid #79839)

• Purpose: Expresses RpBphP1-mCherry-Intersectin1 DHPH domain under CMVd1
• Depositing Lab: Vladislav Verkhusha
• Publication: Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.

Backbone

• Vector backbone: pFC15K
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2700
• Total vector size (bp): 6800
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RpBphP1-mCherry-Intersectin1 DHPH domain
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 4100
• GenBank ID: NCBI Gene rpa1537 KX063613
• Promoter: CMVd1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ggtaggcgtgtacggtgggag
• 3′ sequencing primer: GCA TCA CAA ATT TCA CAA ATA AAG C

Resource Information

• Supplemental Documents:
  • pKA-144
• A portion of this plasmid was derived from a plasmid made by: RpBphP1 gene of R. palustris (NCBI Gene rpa1537) was provided by E. Giraud (Institute for Research and Development, France) A DHPH domain of human intersectin-1 was PCR amplified from a plasmid (Addgene #22278)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry