pLV-TetO-hNGN2-eGFP-Puro
(Plasmid #79823)

• Purpose: Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.
• Depositing Lab: Kristen Brennand
• Publication: Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 10.1016/j.ymeth.2015.11.019.

Backbone

• Vector backbone: Tet-O-FUW
• Backbone size w/o insert (bp): 8387
• Total vector size (bp): 10687
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hNGN2-P2A-eGFP-T2A-PuroR
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• GenBank ID: NM_024019.3
• Entrez Gene: NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
• Promoter: TetO

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: It was modifed from a kind gift from Dr. Sudhof. "Rapid Single-Step Induction of Functional Neurons from Human Pluripotent Stem Cells", Zhang et al.. 2013
• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry